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Protein microarray technology has emerged from the more established technique of DNA microarray technology. Microarrays are simply a technique where individual molecules such as oligonucleotides-/oligodeoxynucleotides, proteins, antibodies and small molecules can be separately spotted onto a surface such as a glass slide and then the surface is analysed for speci c functions or activities (Figure 9.25). DNA microarrays can now be used at densities of tens of thousands of different oligonucleotide probes per square centimetre and can be used for genetic analysis as well as expression analysis at the mRNA level. As previously discussed, mRNA levels do not strictly correlate with protein levels, and therefore protein microarrays have also been developed to probe proteomes in a microarray format. One signi cant problem with protein arrays relative to oligonucleotide arrays is stability it is important to array the proteins without denaturing but maintaining a reasonable density for facile detection of activity. The surfaces are therefore modi ed and engineered to prevent evaporation and drying by the use of sophisticated pads, lms, nano-wells and micro- uidic channels. Arrays used in proteomics take two forms analytical arrays and functional arrays:

The nearly equal amount of cis and trans products formed from 1,5-dimethylcyclohexene is explained by the almost equal degree of hindrance of the homoallylic methyl group with the catalyst surface in the alternate adsorption modes,63,64 Another interesting example is the platinum-catalyzed hydrogenation of isomeric octalins,65-67 If syn addition to the double bond is assumed, in principle, both cis- and trans-decalin are expected to result from 1(9)-octalin, but only the cis isomer from 9(10)-octalin, In contrast with these expectations, the isomers are produced in nearly the same ratio from both compounds, Transformation in the presence of deuterium revealed that most of the products contained three deuterium atoms, This was interpreted to prove that the very slow rate of hydrogenation of 9(10)-octalin [Eq, (11,9)] permits its isomerization to the 1(9) isomer. As a result, most of the products are formed through 1(9)-octalin [Eq, (1 LIO)]:

Figure 10-6: This triangle was created with the Line Tool, using Snap to Grid, with the Grid s visibility enabled with View Grid Show Grid.

The formulas that you develop can also use worksheet functions, but the functions can t refer to cells or named ranges.

(5.19)

Widgets are constructed by passing textual Tk command strings to the Tk module using the and function of the Tk module. The namechan function is used to bind event channel names in the Tk command strings to Limbo channels. The first step in constructing a GUI using the Tk module is to create a 'toplevel' entity on which individual widgets will be created. This is done with the top level function, which returns a reference to a Top level ADT instance. This instance contains an ID for the toplevel widget, as well as a reference to the Image that represents the pixels of the toplevel widget. After creating the toplevel widget, Tk widgets are created by calling the cmd function with a reference to the toplevel widget and a Tk command string, e.g. to create a button widget. These Tk command strings are mostly identical to those used in Tcl/Tk. The peculiarities of Limbo/Tk are described in [32]. There is a command-line utility, tkcmd(1), similar to the wish utility in implementations of Tcl/Tk, for interactively building Tk widgets. It is useful in prototyping Tk widgets before they are implemented in Limbo applications. Events on such a widget can be specified in Tk to be sent on a Tk event name. In order to make such events available to a Limbo program for possible actions, Tk event names can be bound to Limbo channels using the namechan function. The following example creates a simple Tk widget, a solitary button on the screen. Pressing the button causes a string to be sent on a channel:

ff(m2)

lash has a pair of tools the Lasso Tool and the Arrow Tool that can be used to select lines, shapes, groups, symbols, buttons, and other items. The Lasso Tool is primarily used to make free-form selections and to select odd-shaped sections of a drawing. The Arrow Tool is used primarily to select discrete lines, shapes, groups, symbols, buttons, and other items. In combination with the Magnet option and the Shape Recognition options, the Arrow Tool has many unique capabilities not found in any other program. In addition to these tools, the new Subselect Tool, which also looks like an arrow, can be used (in a limited way) to select these items. However, the Subselect Tool is primarily a companion for the Pen Tool, which is introduced at the end of this chapter. We ve chosen to address the Pen Tool among the selection tools because the Pen Tool draws lines by laying down editable points. Additionally, both the Pen Tool and the Subselect Tool are used to manipulate those points, and, thereby, edit lines. Nevertheless, both the Pen Tool and the Subselect Tool are equally useful for selecting and editing all lines and shape, so they re selection tools, too.